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Separation and Analysis of Phosphatidylcholine in Flax Lethin by HPLC

GUAN Ming,HOU Jun-feng,GUZAILINUER·Aerken,AISANJIANG·Aihaiti   

  1. Key Laboratory of Pollution Monitoring and Control, College of Chemistry and Chemical Engineering,Xinjiang Normal University, Ürümqi 830054, China
  • Online:2013-09-25 Published:2013-09-27

Abstract:

Objective: To establish an HPLC method for the separation and analysis of phosphatidylcholin in flax lecithin.
Methods: A Platisil C18 column (150 mm × 4.6 mm, 5 μm) was used for chromatographic separation using acetonitrilemethanol
(50:50, V:V) as mobile phase at flow rate of 1 mL/min. The detection wavelength was set at 215 nm. Results: The
content of phosphatidylcholin in flax lecithin was 73.6%, and the average spiked recovery rate was 103.3%. The precision
RSD for 6 replicate determinations was 0.28%, and the detection limit was 0.25 μg. Conclusion: The method is accurate,
efficient and can provide reliable results.

Key words: high performance liquid chromatography (HPLC), phosphatidylcholine, flax lecithin