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Determination of Polysaccharides from Vinasse by α-Naphthol-Sulfuric Acid Method

ZHANG Shi-xian,LIU Yan,ZHU Bin,JIN Qian,ZENG Qi-hua   

  1. Laboratory of Utilization Research on Characteristic Resources in Qianbei, School of Chemistry and Chemical Engineering, Zunyi Normal College, Zunyi 563002, China
  • Online:2013-09-25 Published:2013-09-27

Abstract:

Objective: To extract polysaccharides from vinasse (byproduct of Chinese liquor making) and to establish a
simple and reliable determination method. Methods: Vinasse polysaccharides were extracted by an enzymatic method,
and α-naphthol-sulfuric acid method was used for the determination of polysaccharide content. Results: The maximum
absorption wavelength was 560 nm. There was a good linear relationship between absorbance and glucose concentration in
the range from 6 to 36 μg/mL (R2 = 0.9975). The average spiked recovery rate was 100.65%. The content of polysaccharides
from vinasse (caculated as glucose) was 10.6% (RSD = 4.0%). Conclusion: The α-naphthol-sulfuric acid method is a simple
and reliable assay with good reproducibility for determining polysaccharides from vinasse.

Key words: vinasse;polysaccharides;&alpha, -naphthol-sulfuric acid method