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Inhibitory Effect of Eriodictyol on Free Radical-induced Damage to Biological Macromolecules and Its Cytotoxicity on HepG2 Cells

ZHANG Yi-fan,LIU Gong-guan,LIU Qian,LIU Xue-bo*   

  1. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China
  • Online:2013-09-15 Published:2013-09-27
  • Contact: LIU Xue-bo

Abstract:

The in vitro free radical-scavenging activity of eriodictyol was evaluated against DPPH and hydroxyl free radicals.
The protective effect of eriodictyol on free radical-induced damage to protein, lipid and DNA were evaluated based on oxidation
models of BSA, rat liver tissue and plasmid DNA induced by AAPH and its cytotoxicity on HepG2 cells was assessed by MTT
assay. The results showed that eriodictyol in the concentration range of 25 to 200 μmol/L had strong scavenging activity against
DPPH free radical with a median inhibitory concentration (IC50) of 95.7 μmol/L. However, the scavenging activity on hydroxyl
free radical was weaker. The inhibitory effect of eriodictyol on oxidative damage to protein, lipid and DNA induced by free
radicals was in a dose-dependent manner. Similarly, eriodictyol could significantly reduce the viability of HepG2 cells in a
dose-dependent manner. Therefore, eriodictyol has the capability to scavenge DPPH free radicals and inhibit macromolecular
damage induced by free radicals. It also can significantly reduce the viability of HepG2 cells.

Key words: eriodictyol, free radical, protein damage, lipid peroxidation, DNA strand breakage, cytotoxicity

CLC Number: