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Identification of Sterile Endophytic Fungus Isolated from Salicornia bigelovii

WANG Xiao-min,WANG Hui,LIU Tian-xing,XIN Zhi-hong*   

  1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
  • Online:2013-09-15 Published:2013-09-27
  • Contact: XIN Zhi-hong

Abstract:

The sequences of 18S rDNA and ITS gene of a fungal strain named Sal54 isolated from Salicornia bigelovii
were cloned and sequenced, and a 18S rDNA gene sequence of 1715 bp in length and an ITS gene sequence of 544 bp in
length were obtained. The accession number of the ITS gene sequence in GenBank was JX292134. Homology analysis was
conducted. A phylogenetic tree was constructed by using these sequences and the neighbor-joining method. Phylogenetic
analysis revealed that the isolated strain was identified as Phoma betae.

Key words: sterile fungi, identification, 18S rDNA gene, ITS gene, phylogenetic analysis

CLC Number: