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Purification and Molecular Mass Determination of Lily Polysaccharides

CHEN Zhi-gang,ZHU Quan,WANG Fen   

  1. Key Laboratory of Agricultural and Animal Products Processing and Quality Control, Ministry of Agriculture,
    College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
  • Online:2013-09-15 Published:2013-09-27
  • Contact: CHEN Zhi-gang

Abstract:

Microwave-assisted hot-water extraction method was used to extract lily polysaccharides. Fractions LLPS-1,
LLPS-2 and LLPS-3 were isolated from the aqueous polysaccharide extract and purified through deproteinizaiton by Sevag’s
method, dialysis, DEAE-Sepharose Fast Flow chromatography and Sephadex G-100 column chromatography. Results from
coomassie blue staining, UV and high-performance gel permeation chromatography (HPGPC) showed that the purified
product contained neither protein or nucleic acid. The molecular weights of LLPS-1, LLPS-2 and LLPS-3 were 350.5,
403.3 kD and 146.2 kD, respectively.

Key words: lily polysaccharides, purification, column chromatography, HPGPC, molecular weight

CLC Number: