Protective Effect of Polysaccharide from Ganoderma atrum against Oxidative Stress-induced Liver Injury in Type Ⅱ Diabetic Rats

doi:10.7506/spkx1002-6630-201321060

Abstract

Abstract:

Objective: To explore the protective effect of polysaccharide from Ganoderma atrum against oxidative stressinduced
liver injury in type Ⅱdiabetic rats. Methods: An animal model of type Ⅱ diabetes was developed by feeding high
fat diet to rats coupled with an injection of STZ at low dose (30 mg/(kg·d)). Type Ⅱ diabetic rats were randomly divided
into three groups: diabetic control group, polysaccharide-treated (100 mg/(kg·d)) diabetic group and NAC (N-acetyl-Lcysteine,
100 mg/(kg·d))-treated diabetic group. Eight normal rats fed on standard normal chow diet served as a non-diabetic
control group. At the end of 4 weeks experimental period, liver function (ALT and AST), liver index, liver glycogen
and antioxidant enzyme activities (SOD, GSH-Px and CAT) as well as lipid peroxidation (MDA) were measured. Bax
and Bcl-2 mRNA expression levels were analyzed by RT-PCR. Results: Ganoderma atrum polysaccharide significantly
improved liver function and liver index, and also increased glycogen content and antioxidant enzyme activities as well as
decreased MDA content in liver of diabetic rats. Moreover, Bcl-2 expression was promoted whereas Bax expression was
inhibited. Conclusion: Polysaccharide from Ganoderma atrum (100 mg/(kg·d)) may improve the liver function of type
Ⅱ diabetic rats through antioxidant effect.

Key words: polysaccharide from Ganoderma atrum, oxidative stress, type Ⅱ diabetes, antioxidant, Bax, Bcl-2

CLC Number:

R575.5

LIN Su-li，ZHU Ke-xue，SONG Dan，NIE Shao-ping*，XIE Ming-yong. Protective Effect of Polysaccharide from Ganoderma atrum against Oxidative Stress-induced Liver Injury in Type Ⅱ Diabetic Rats[J]. FOOD SCIENCE, 2013, 34(21): 302-305.

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