Effect of Chlorogenic Acid on Damaged Intestinal Mitochondria in Rats

doi:10.7506/spkx1002-6630-201321070

Abstract

Abstract:

Objective: To explore if chlorogenic acid (CHA) could ameliorate intestinal mitochondrial damage in rats. Methods:
Intestinal mitochondria of rats were treated with hydrogen peroxide. The mitochondrial swelling, membrane potential, MDA content,
T-SOD and GSH-Px activities, and the enzymatic activities of mitochondrial respiratory chain complexes Ⅰ, Ⅳ, and Ⅴ were
evaluated. Results: CHA significantly inhibited mitochondrial swelling caused by hydrogen peroxide and reduced cell membrane
potential. CHA (0.032 mg/mL and 0.064 mg/mL) significantly decreased the concentration of MDA when compared with the control
group. The activities of T-SOD, GSH-Px and complex Ⅰ in the CHA group (0.064 mg/mL) were significantly increased when
compared with the control group. Conclusion: CHA can attenuate mitochondrial damage induced by hydrogen peroxide through
increasing the activities of mitochondrial respiratory chain complex enzymes and antioxidant enzymes.

Key words: chlorogenic acid, mitochondria, oxidative damage, respiratory chain complex

CLC Number:

TS201.4

ZHOU Li-li，RUAN Zheng*，ZHOU Yan，YANG Yu-hui，LI Xiao-lan， JIANG Min. Effect of Chlorogenic Acid on Damaged Intestinal Mitochondria in Rats[J]. FOOD SCIENCE, 2013, 34(21): 349-353.

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