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MTT Colorimetric Method for Lactobacillus helveticus MB 2-1 Viable Cell Counting

YANG Pei-jie,LI Teng,CHEN Xiao-hong,LI Wei,DONG Ming-sheng   

  1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
  • Online:2013-10-25 Published:2013-09-28

Abstract:

The feasibility of MTT colorimetric method for Lactobacillus helveticus MB2-1 viable cell counting
was evaluated, and the wavelength, MTT dosage, DMSO dosage and incubation time were optimized. Results
showed that the OD value was linear with the cell concentration (R2 > 0.99) at cell concentrations between
107 CFU/mL and 109 CFU/mL. The best working procedure was described as following: 1.0 mL of bacterial suspension
reacted with 0.2 mL of MTT solution for 1 h min at 40 ℃. After centrifugation at 12000 r/min for 10 min, 1.0 mL of DMSO
was added to dissolve the precipitate. OD510nm values were measured. This study indicates that the MTT colorimetric method
can be applied for lactobacillus viable cell counting.

Key words: MTT colorimetric method, Lactobacillus helveticus MB 2-1, viable cell counting, optimization