Effect of L-malate on Gene Expression of Carrier Proteins and Antioxidant Enzymes in Rats

doi:10.7506/spkx1002-6630-201323056

FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (23): 276-281.doi: 10.7506/spkx1002-6630-201323056

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Effect of L-malate on Gene Expression of Carrier Proteins and Antioxidant Enzymes in Rats

ZENG Xiao-cong1,2，WU Jun-lin2，XIAO Chun2，WU Qing-ping2，XU Ming-fang1，ZHANG Ju-mei2,*

1. College of Life Science and Technology, Jinan University, Guangzhou 510006, China；
2. State Key Laboratory of Applied Microbiology, South China (The Ministry-Province Joint Development), Guangdong Provincial
Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong
Institute of Microbiology, Guangzhou 510070, China

Received:2013-07-18 Revised:2013-11-04 Online:2013-12-15 Published:2014-01-03
Contact: ZHANG Ju-mei E-mail:Zhangjm926@126.com

Abstract

Abstract:

To investigate the antioxidant mechanism of L-malate on mitochondria, real time RT-PCR was used to analyze
the gene expressions of two carrier proteins (AGC and OMC) in the inner membrane and two antioxidant enzymes (CAT
and GSH-Px) related to the malate-aspartate shuttle in rat liver and heart mitochondria. The mRNA expressions of these
proteins were examined by real time RT-PCR in hearts and livers of SD rats treated with L-malate. The results indicated that
the mRNA expressions of AGC, OMC, CAT and GSH-Px in the heart of rats treated with L-malate were 1.25, 1.39, 1.12, 1.01
times as much as those in the control group. The mRNA expression of AGC, OMC, CAT and GSH-Px in the liver of rats
treated with L-malate was 1.33, 1.02, 1.25, 0.94 times as much as those in the control group. These results imply that L-malate
might increase the antioxidant capacity of mitochondria by enhancing the mRNA expression of the proteins involved in the
malate-aspartate shuttle and the antioxidant enzymes.

Key words: mitochondria antioxidant capacity, malate-aspartate shuttle, real-time reverse transcription polymerase chain reaction (real-time RT-PCR)

CLC Number:

Q812

ZENG Xiao-cong1,2，WU Jun-lin2，XIAO Chun2，WU Qing-ping2，XU Ming-fang1，ZHANG Ju-mei2,*. Effect of L-malate on Gene Expression of Carrier Proteins and Antioxidant Enzymes in Rats[J]. FOOD SCIENCE, 2013, 34(23): 276-281.

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Effect of L-malate on Gene Expression of Carrier Proteins and Antioxidant Enzymes in Rats

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