FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (23): 221-227.doi: 10.7506/spkx1002-6630-201323046

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Strain Screening of Pleurotus nebrodensis and Optimization of Submerged Culture Conditions for Biomass Prodution

JI Hong1,2, ZHAO Li-ming1   

  1. 1. Department of Management and Economics, Tianjin University, Tianjin 300072, China;
    2. School of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China
  • Received:2013-06-22 Revised:2013-11-01 Online:2013-12-15 Published:2014-01-03
  • Contact: JI Hong E-mail:jihong8848@126.com

Abstract:

Pleurotus nebrodensis Tianshan No.2 from Wuan, Hebei province was selected as the fastest-growing of
19 strains. The culture medium for submerged fermentation of strain Tianshan No.2 were optimized for the maximum
biomass (dry cell weight, DCW) production using an orthogonal array design L9 (34), and three culture conditions including
temperature, shaking speed and time were optimized by response surface analysis based on a three-variable, three-level Box-
Behnken experimental design. The results showed that the optimal medium composition (per 100 mL) was 3.50 g of soluble
starch, 0.40 g of beef extract, 0.25 g of monopotassium phosphate and 0.25 g of magnesium sulfate. The mycelia biomass
of strain Tianshan No.2 was 1.6004 g/100 mL after 7.5 d (180 h) of shaking culture at 26 ℃ with a shaking speed of 160 r/min
using the optimized medium.

Key words: Pleurotus nebrodensis, submerged fermentation, mycelia biomass, orthogonal array design, response surface methodology

CLC Number: