Isolation and Purification of Antioxidative Polysaccharides from Spirulina platensis

doi:10.7506/spkx1002-6630-201323018

Abstract

Abstract:

Crude polysaccharides with antioxidant activity were obtained from S. platensis through extraction with a hot
alkali solution followed by precipitation with 95% ethanol. The scavenging activities against hydroxyl and superoxide
anion free radicals of the crude polysaccharides were evaluated. Further studies were carried out to separate and purify
the crude polysaccharide extract by chromatography on a DEAE-52 cellulose column and measure the molecular masses
and IR spectroscopic features of fractions. The crude polysaccharides extracted from S. platensis had a potent free radical
scavenging activity, and the scavenging capacity was (37.01 ± 1.58) U/mL and (47.84 ± 2.46) U/mL for hydroxyl and
superoxide anion radicals when used at concentrations of 0.6 mg/mL and 0.4 mg/mL, respectively. Four water-soluble acidic
fractions designated as SP1-2, SP2-1, SP3-1 and SP4-1 were obtained with molecular weight of 4.18 × 104, 5.11 × 104, 2.80 ×
104 D and 3.76 × 104 D. The infrared (IR) spectrum of SP3-1 showed the characteristic absorption of glycoprotein. These
results indicate that S. platensis polysaccharides may be used as an effective antioxidant in functional foods.

Key words: Spirulina platensis, polysaccharide, hydroxyl radical, superoxide anion radical, isolation and purification

CLC Number:

S931.9

CAI Bing-na1，CHEN Chun-xin2，CHEN Xin2，SUN Hui-li1,3，CHEN Yan-yun2，PAN Jian-yu1,*. Isolation and Purification of Antioxidative Polysaccharides from Spirulina platensis[J]. FOOD SCIENCE, 2013, 34(23): 83-87.

References

[1] Sarma A P, Petar P, Murthy S D S. Spirulina as a source of single cell protein[J]. Vegetos, 2008, 21(1):35-45.

[2] 顾林娣，陈坚，章宗涉. 螺旋藻提取物对护肤效果的研究[J]. 上海师范大学学报(自然科学版)，1997，26（2）：78-83.

[3] 张许昌，杨平顺. 天然螺旋藻护肤护发性能的研究[J].日用化学工业，1998（4）：23-28.

[4] 朱晓龙，李虹. 螺旋藻提取物在化妆品中的应用[J]. 云南民族学院学报(自然科学版)，1998，7（2）：17-22.

[5] 刘玉兰，牟孝硕，颜鸣，等. 螺旋藻多糖的抗衰老作用[J].中国药理学通报，1998,14（4）：362-364.

[6] 李羚，高云涛，戴云，等. 螺旋藻及螺旋多糖体外清除活性氧及抗氧化作用研究[J].化学与生物工程，2007,24（3）：55-57.

[7] Hosseini S M, Khosravi-Darani K, Mozafari M R. Nutritional and medical applications of spirulina microalgae[J]. Mini reviews in medicinal chemistry, 2013, 13(8):1231-1237.

[8] Chaiklahan R, Chirasuwan N, Triratana P, et al. Polysaccahride extraction from Spirulina sp. and its antioxidant capacity[J]. International Journal of Biological Macromolecules, 2013,58:73-78.

[9] Niki E. Assessment of antioxidant capacity in vitro and in vivo[J]. Free Radical Biology and Medicine, 2010, 49(4):503-515.

[10] ZHANG Zhongshan, WANG Xiaomei, YU Shuichi, et al. Synthesized oversulfated and acetylated derivatives of polysaccharide extracted from Enteromorpha linza and their potential antioxidant activity[J]. International Journal of Biological Macromolecules, 2011,49(5):1012-1015.

[11] Vern L B, Merril S, Norella M P, et al. Handbook of theories of aging[M]. 2nd ed. New York: Springer Publishing Company, 3-24.

[12] Saha S K, Brewer C F. Determination of the concentrations of oligosaccharides, complex type carbohydrates, and glycoproteins using the phenol sulfuric-acid method[J]. Carbohydrate Research, 1994, 254:157-167.

[13] WANG Shaoyun, Agyare K, Damodaran S. Optimisation of hydrolysis conditions and fraction of peptide cryprotectants from gelatin hydrolysate[J]. Food Chemistry, 2009,115(2):620-630.

[14] Kozarski M, Klaus A, Niksic M, et al. Antioxidative and immunomodulating activities of polysaccharide extracts of the medicinal mushrooms Agaricus bisporus, Agaricus brasiliensis, Ganoderma lucidum and Phellinus linteus[J]. Food Chemistry, 2011, 129(4):1667-1675.

[15] Ananthi S, Raghavendran H R B, Sunil A G, et al. In vitro antioxidant and in vivo anti-inflammatory potential of crude polysaccharide from Tubinaria ornate ( marine brown algae)[J]. Food and Chemical Toxicology, 2010,48(1):187-192.

[16] Pawlaczyk I, Czerchawski L, Pelecki W, et al. Polyphenolic-polysaccharide compounds from selected medicinal plants of Asteraceae and Rosaceae families: chemical characterization and blood antiocoagulant activity[J]. Carbohydrate Polymers, 2009, 77(3):568-575.

[17] HUANG Dejian, QU Boxin, PRIOR R L. The chemistry behind antioxidant capacity assays[J]. Journal of Agricultural and Food Chemistry, 2005,53(6):1841-1856.

[18] Barahona T, Emcinas M V, Mansila A, et al. A sulfated galactan with antioxidant capacity from the green variant of tetrasporic Gigartina skottsbergii (Gigartinales, Rhodophyta)[J]. Carbohydrate Research, 2012,347:114-120.

[19] Karadag A, Ozcelik B, Saner S. Review of methods to determine antioxidant capacities[J]. Food Analytical Methods, 2009, 2(1):41-60.

[20] 傅裕. 体外抗氧化功能评价方法研究进展[J]. 肉类研究，2010,（11）：41-46.