Fractional Purification and Structural Analysis of Polysaccharides Extracted from Camellia oleifera Seed Cake

doi:10.7506/spkx1002-6630-201323021

Abstract

Abstract:

In this study, we conducted fractional purification and structure analysis of polysaccharides (CSPs) extracted
from Camellia oleifera (C. oleifera) seed cake with the aim to provide experimental data for subsequent studies on the
structure-function relationship of CSPs. Methods: After removal of proteins, the crude CSPs were dialyzed, and then
fractionated and purified by DEAE-Sephadex A-25 anion exchange resin column chromatography. UV spectroscopy,
Sephacryl S-200 column chromatography and HPLC were used to detect the purity of the purified polysaccharides. A precolumn
derivatization HPLC method was used to analyze the monosaccharide composition. Infrared spectroscopy and a
nuclear magnetic resonance scanner were then used to study the configuration and structures of the purified CSPs. The
fractional purification led to three polysaccharides, namely CSP1, CSP2 and CSP3, as the main polysaccharides of CSP.
Both CSP1 and CSP2 were found to be neutral heteropolysaccharides whereas CSP3 a homogeneous, acidic one. CSP1was
composed of glucose, mannose and rhamnose with a molar ratio of 6.29:1.00:0.15; CSP2 was composed of monosaccharides
glucose, mannose and galactose with amolar ratio of 3.13:1.00:0.03; and CSP3 was composed of monosaccharides glucose,
mannose, galactose, arabinose, galactose, rhamnose, galacturonic acid and a small amount of rhamnose with a molar ratio of
0.42:1.00:1.09:0.96:0.43:0.19.

Key words: Camellia oleifera seed cake, polysaccharides, fractional purification, structure

CLC Number:

Q929.2

LIU Xiao-ru，ZHANG Li-mei，HU Jiang-ning，LIU Rong，LI Hong-yan，FAN Ya-wei，LI Jing，DENG Ze-yuan*. Fractional Purification and Structural Analysis of Polysaccharides Extracted from Camellia oleifera Seed Cake[J]. FOOD SCIENCE, 2013, 34(23): 96-102.

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