Separation and Identification of A-type and B-type Dimers of Procyanidins from Peanut Skin by RP-HPLC-ESI-MS/MS

doi:10.7506/spkx1002-6630-201323030

Abstract

Abstract:

A-Type and B-type oligomeric proanthocyanidins were prepared from the 70% ethanol extract of peanut skin
by purification on AB-8 macroporous adsorption resin and two fractions PSP-3 and PSP-5 were separated and identified
by reversed phase high-performance liquid chromatography-tandem mass spectrometry (RP-HPLC-MS/MS). Peanut skin
procyanidins (PSP) with a purity of over 98.0% and a recovery of 9.8% were obtained after the purification. PSP-3 was
composed of 2 B-type procyanidolic dimers and PSP-5 was composed of 4 A-type procyanidolic dimers.

Key words: peanut skin, A-type procyanidins, B-type procyanidins, reverse phase high performance liquid chromatography combined with tandem mass spectrometry (RP-HPLC-MS/MS)

CLC Number:

TS201.2

CHEN Yang，WANG Bing，YU Jia-nan，LI Bao-li，WEN Yun，LIU Rui*，TAN Jun*. Separation and Identification of A-type and B-type Dimers of Procyanidins from Peanut Skin by RP-HPLC-ESI-MS/MS[J]. FOOD SCIENCE, 2013, 34(23): 142-146.

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