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Negative Pressure Cavitation-Assisted Extraction and Purification of Proanthocyaidins from Ginkgo biloba Leaves

WANG Yan-qin,XU Li,CAO Fu-liang,WANG Wei-fan,ZHAO Feng-yi,YANG Shi-long,LEI Meng   

  1. 1. College of Science, Nanjing Forestry University, Nanjing 210037, China;
    2. Advanced Analysis and Testing Center, Nanjing Forestry University, Nanjing 210037, China;
    3. College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China
  • Online:2013-12-25 Published:2014-01-03

Abstract:

This study dealt with the optimization of negative pressure cavitation-assisted extraction of proanthocyandins
from Ginkgo biloba leaves. Using the extraction efficiency of proanthocyandins as the response value, response surface
and contour plots were drawn, and the optimum extraction conditions were determined as follows: extraction pressure of
-0.05 MPa, solid-to-liquid ratio of 1:10.1 (g/mL) and ethanol concentraction of 50%. Under these extraction conditions, the
extraction efficiency of proanthocyandins was 5.54%. The crude extract of proanthocyanidins was purified with macroporous
adsorption resin. Our static adsorption and dynamic desorption experiments indicated that AB-8 was the best resin for
proanthocyandin purification, and the optimum chromatographic conditions were found as follows: 10 h adsorption at 30 ℃,
resulting in an adsorption quantity of 8.74 mg/g, and desorption with absolute ethanol at a flow rate of 2 mL/min, providing
a desorption efficiency of 80.5%.

Key words: Ginkgo biloba leaves, proanthocyaidin, negative pressure cavitation, extraction, purification