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Ultrasonic-Assisted Aqueous Two-Phase Extraction and Antimicrobial Activity of Sweet Corncob Polyphenols

MA Yong-qiang,JING Li-rong,LIU Xiao-fei,ZHANG Na,LIU Xiao-ning   

  1. Key Laboratory for Food Science and Engineering, Harbin University of Commerce, Harbin 150076, China
  • Online:2013-12-25 Published:2014-01-03

Abstract:

One-factor-at-a-time and orthogonal array designs were applied to establish for optimal conditions for ultrasonicassisted
aqueous two-phase extraction of polyphenols from sweet corncob. The effects of four process parameters including
acetone concentration, ammonium sulfate dosage, extraction time and solid-to-liquid ratio on the yield of polyphenols were
investigated. The optimal extraction conditions were found as follows: an aqueous two-phase system formed by adding 0.175 g/mL
of ammonium sulfate to 60% aqueous acetone, solid-to-solvent ratio of 1:20 (g/mL), and 25 min extraction. The yield of
polyphenols in extracts under these conditions was 4.41%. The polyphenols extracted from sweet corncob had significant
antibacterial activity against Escherichia coli, Bacillus subtilis and Staphylococcus white.

Key words: sweet corncob, polyphenols, ultrasonic-assisted extraction, antibacterial activity