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Determination of α-solanine in Potato Using Liquid Chromatography-Mass Spectrometry

WU Hui-min,ZENG Jing,LI Mei,LIU De-ming,DU Yu,XIONG Xing-yao,ZENG Jian-guo   

  1. 1. College of Pharmacy, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;
    2. Pre-State Key Laboratory for Germplasm Innovation and Resource Utilization of Crops, Hunan Agricultural University,
    Changsha 410128, China;3. Hunan Engineering Research Center of Botanical Extract, Changsha 410331, China
  • Online:2013-12-25 Published:2014-01-03

Abstract:

Objective: To establish a sensitive method to determine micro amounts of α-solanine in potato using liquid
chromatograph-mass spectrometry (LC-MS). Methods: Potato samples were extracted with 1% formic acid-methanol
(1:1, V/V) in combination with ultrasonic assistance for 50 min. Separation of the extracts was performed on a C18
column (2.1 mm × 150 mm, 5 μm). The mobile phase consisted of acetonitrile and 0.1% formic acid. Gradient elution was
performed at column temperature of 35 ℃ with a flow rate of 0.2 mL/min. The pooled eluate was detected by electrospray ionization
mass spectrometry. Results: The regression coefficient of α-solanine was 0.9991 in the range of 7–460 μg/kg. The average recovery
rate was 93.6%. The precision as repeatability and reproducibility were 1.13% and 2.59%, respectively. Conclusion: This method is
simple, accurate, repeatable and applicable to determine micro amounts of α-solanine in potato.

Key words: potato (Solanum tuberosum L.);solanine;&alpha, -solanine;liquid chromatography-mass spectrometry (LC-MS)