Abstract:

A method was developed for the simultaneous determination of BaP, BHA, BHT, TBHQ, DBP and DEHP in edible
oil by gel permeation chromatography and high performance liquid chromatography (GPC-HPLC). Samples were purified by GPC
after pretreatment, then separated on a reversed-phase C18 column with acetonitrile and water as the mobile phase in a gradient
elution mode. The target compounds were detected by UV-tandem fluorescence detector. Under the preferred test conditions, the
linear ranges of the method were 0.0002–0.2 mg/L for BaP, 1–500 mg/L for BHA, BHT and TBHQ, and 0.1–100 mg/L for DBP
and DEHP, with correlation coefficients of more than 0.9980. The spiked recovery rates were 82.0%–103.7% with relative standard
deviations (RSD, n = 6) of 3.2%–7.5%. The developed method is simple and accurate, and can be used for the simultaneous
determination of BaP, BHA, BHT, TBHQ, DBP and DEHP in edible oil samples.

Key words: gel permeation chromatography, reversed-phase high performance liquid chromatography, edible oil