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Determination of Oxidative DNA Damage Induced by Quinoxaline in Apostichopus japonicas Tissue Using High Performance Liquid Chromatography

JIANG Xiangyang1, ZOU Rongjie1,2, XU Yingjiang1, SONG Xiangjun1, GONG Xianghong1,LIU Huihui1, TIAN Xiuhui1, SUN Guohua1, LIU Yun3, AN Honghong3, ZHANG Xiuzhen1,*   

  1. 1. Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Shandong Marine Resource and Environment Research
    Institute, Yantai 264006, China; 2. Yantai Shanshui Seafood Co. Ltd., Yantai 264006, China;
    3. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
  • Online:2015-03-25 Published:2015-03-17
  • Contact: ZHANG Xiuzhen

Abstract:

8-Hydroxy-2’-deoxyguanosine (8-OHdG) is one of the main modified products from oxidative DNA damage
when deoxyguanosine (dG) is oxidized by a large amount of active oxygen species (ROS). Once 8-OhdG has avoided to
be repaired within the body, it maybe the initiation factor causing mutagenic, teratogenic and carcinogenic effect. 8-OHdG
is a metabolic end product that is stable within the body. It is not formed by non-DNA oxidation pathway outside the cell.
The content of 8-OHdG can reflect the degree of oxidative damage in DNA. Thus, it is important to determine the content
of 8-OHdG in order to evaluate the interaction between oxidative damage, oxidative stress and DNA damage in the body.
The quinoxaline-induced DNA oxidative damage in Apostichopus japonicas tissues was determined by high performance liquid
chromatography (HPLC) in this study. Sea cucumber tissue treated with quinoxalines at different concentrations and for different
time periods were collected. DNA were extracted and treated enzymatically, and then 8-OHdG as an indicator of DNA oxidative
damage was measured and analyzed by HPLC. The results showed that the HPLC-UV method displayed a wide applicability,
stability and accuracy, high sensitivity, less samples and fast analysis. The 8-OHdG content remarkably increased along with
increasing concentration and processing time of mequindox. The dose- and time-dependent response relationship existed,
which were a continual process. The oxidative damage to DNA caused by quinocetones significantly increased with increasing
concentration (P < 0.05). It was indicated that there was obvious genetic toxicity resulting from oxidative DNA damage caused by
mequindox and quinocetone in sea cucumber. Thus, it is necessary to control strictly quinoxalines applied in sea cucumber.

Key words: quinoxaline, Apostichopus japonicas, oxidative DNA damage, high performance liquid chromatography (HPLC)

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