Abstract:

The seed oil of Akebia trifoliate was prepared by reflux and ultrasonic extraction, and its chemical constituents
were measured by gas chromatography-mass spectrometry. Influence of preparation methods on the extraction yield and
chemical components of oil were investigated. The results showed that the extraction yields of oil by petroleum ether reflux
extraction and petroleum ether, alcohol, ethyl acetate, acetone ultrasonic extraction were 36.0%, 38.6%, 28.0%, 30.8%, and
36.4%, respectively. Fatty acid contents of five oils were 69.69%, 83.51%, 89.78%, 69.33%, and 57.54%, and the contents
of unsaturated fatty acid were 45.25%, 51.80%, 37.54%, 42.21%, and 43.31%, respectively. The main fatty acids in the seed
oils were hexadecanoic acid, oleinic acid, and linoleic acid. Extraction yield and the contents of fatty acid and unsaturated
fatty acids in the seed oil were higher by petroleum ether ultrasonic extraction.

Key words: Akebia trifoliate, oil, chemical constituent, gas chromatography-mass spectrometry (GC-MS)