Abstract:

In this study, the pepsin-catalyzed hydrolysis of fresh pig skin was optimized by response surface methodology
(RSM) based on Box-Behnken design. The antioxidant activity of the resulting hydrolysates was studied by scavenging
capacities against superoxide anion radical, hydroxyl radical and hydrogen peroxide and DNA damage protection activity.
The optimal conditions for the enzymatic hydrolysis of porcine skin were determined to be hydrolysis at 41.15 ℃ for 1.65 h
with an enzyme amount of 0.25% and solid/liquid ratio of 1:2. Under these conditions, the IC50 of the hydrolysate for
scavenging of superoxide anion, hydroxyl radicals and hydrogen peroxide and for DNA damage protection were 20.19, 6.36,
0.65, and 1.86 mg/mL, respectively.

Key words: pig skin, collagen, pepsin, antioxidant activity, DNA damage