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Damage of Saccharomyces cerevisiae Cell and Permeability Induced by Pulsed Light Irradiation

ZHANG Bai-qing,WANG Rong   

  1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China
  • Published:2013-10-28

Abstract:

The study was designed to explore damage of Saccharomyces cerevisiae cell and the change of permeability after pulsed light treatment as a non-thermal physical sterilization technology on yeast suspension. When the light flux was 1790.37 mJ/cm 2, the leakage of MDA was 8.01 nmol/mL and the SOD activity was reduced to 53.02%. The AKP activity was 1.17 U/L. In addition, the contents of Na+ and K+ were changed. The transmission electron microscopy (TEM) showed the deformation and rupture of Saccharomyces cerevisiae cells. From these results, we draw the conclusion that Saccharomyces cerevisiae cells were damaged, which led to leakage of cell contents and changes of ion channels or even cell death.

Key words: pulsed light, Saccharomyces cerevisiae, cell damage, cell permeability

CLC Number: