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Optimization of Synthetic Complete Medium for Enhanced Nattokinase Production by Genetically Engineered Bacillus licheniformis

ZHAO Xinyu1,2, CHEN Yangyang2, CHEN Jingbang2, CAI Dongbo2, WEI Xuetuan1,2,*   

  1. 1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;
    2. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China
  • Online:2016-04-15 Published:2016-04-13

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Abstract:

The nattokinase-producing genetically engineered strain, Bacillus licheniformis BL10 (pP43SNT-SsacC) was
used in this study, and the synthetic complete medium composition for nattokinase production by the strain was optimized
using combination of single factor and orthogonal tests. The maximum nattokinase activity of 25.59 FU/mL, which was
6 times higher than that (4.27 FU/mL) before optimization, was obtained using a medium consisting of 30 g/L glucose, 30 g/L
NaNO3, 20 g/L sodium glutamate, 15 g/L sodium citrate, 0.5 g/L MgSO4·7H2O, 1.5 g/L K2HPO4·3H2O and 0.5 g/L CaCl2 at
pH 7.2. The specific activity of nattokinase in the synthetic complete medium was improved by 3 folds when compared with
that in semi-synthetic medium. In the present study, both the activity and purity of nattokinase were improved obviously by
using the optimized synthetic complete medium.

Key words: nattokinase, genetically engineered Bacillus licheniformis, synthetic complete medium, fermentation optimization

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