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Optimization of Cell Wall Breakdown by Vacuum Air Current Technique for Synephrine Extraction from Abscising Fruits of Citrus tankan Using Response Surface Methodology

LIU Mouquan1, KONG Meilan1, LIU Zhicong1, ZHANG Fuping1, CHEN Debin2   

  1. 1. College of Life Science and Food Technology, Hanshan Normal University, Chaozhou 521041, China;
    2. Guangdong Fuwei Fruits & Nuts Manufacturing Co. Ltd., Shantou 515011, China
  • Online:2016-04-25 Published:2016-04-13

Abstract:

In order to improve the extraction efficiency of synephrine from abscising fruits of Citrus tankan and
simultaneously protect the morphological integrity of tissues, vacuum air current technique was applied in cell wall
breakdown for subcritical water extraction of synephrine. The effect of two tissue protectants on the percentage of broken
fruits and extraction efficiency was examined. The experimental conditions for cell wall breakdown were optimized to
maximize the extraction efficiency of synephrine by Box-Behnken design and response surface methodology (RSM) based
on single-factor experiments. Sequential soaking in 0.3 g/100 mL sodium alginate solution for 20 min and 0.15 g/100 mL
calcium chloride solution for another 60 min provided optimal protection of the tissue. The optimum conditions for cell wall
breakdown were determined as follows: decompression temperature, 100 ℃; pressure difference, 116 kPa; retention time,
21 min; and 3 decompression cycles. Under these pretreatment conditions, the extraction yield of synephrine was 69.42%
and the percentage of broken fruits was 33.21%, which were increased by 26.94% and decreased by 12.07% compared with
the control group without this pretreatment, respectively.

Key words: Citrus tankan, abscising fruits, vacuum air current for plant cell wall breakdown (VAPB), synephine

CLC Number: