FOOD SCIENCE ›› 2016, Vol. 37 ›› Issue (20): 172-176.doi: 10.7506/spkx1002-6630-201620029

• Safety Detection • Previous Articles     Next Articles

Preparation and Application of Multi-Target Immunoaffinity Columns with Polystyrene-Divinylbenzene (PS-DVB) as CarrierL

ZHANG Jianing, DING Ke, HAN Tao, CHEN Xiangning   

  1. Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide, Beijing Laboratory of
    Food Quality and Safety, Food Science and Engineering College, Beijing University of Agriculture, Beijing 102206, China
  • Received:2016-10-27 Revised:2016-10-27 Online:2016-10-25 Published:2016-12-01
  • Contact: DING Ke

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Abstract:

Three kinds of polystyrene-divinylbenzene (PS-DVB) resins were selected to prepare immunoaffinity columns
(IAC) through nitration, amination, activation, and antibody coupling for the simultaneous purification of four Fusarium
toxins, zearalenone, deoxynivalenol, T-2 and HT-2 toxins. Among these, the coupling ratio of Cleanert PS-type PS-DVB
IAC was 97.64% and the absorption of its blank column for samples was the lowest. The recovery of this IAC column at
three spiked concentration levels was in the range of 66.16%–112.80%, and the relative standard deviation was between
3.09% and 12.25%. It can be applied to purify actual samples.

Key words: polystyrene-divinylbenzene (PS-DVB), immunoaffinity columns (IAC), Fusarium toxinsn

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