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• Nutrition & Hygiene •     Next Articles

Transepithelial transport of milk-derived peptide QEPV in vitro and in vivo

1, 1,Zhi-Cai CHENGpeng Shen1, 1,David XianLi1,Shao-Hui ZHANG   

  • Received:2016-09-08 Revised:2017-09-29 Online:2017-12-15 Published:2017-12-07
  • Contact: Shao-Hui ZHANG E-mail:yuflili@163.com

Abstract: Abstract: Fermented milk benefits human health in many respects. Gln-Glu-Pro-Val (QEPV) is a peptide produced through the enzymatic hydrolysis of the milk-derived peptide Gln-Glu-Pro-Val-Leu. QEPV elicits immunomodulating effects on lymphocytes and functions as a bioactive peptide. In this research, the transepithelial transport of QEPV was investigated in vitro and in vivo by using Caco-2 cell monolayer and mouse models. Results showed that QEPV exhibited good stability and it could integrally cross the Caco-2 cell monolayer in vitro. A concentration limit within 3.00mg/mL was seen for the transepithelial transport of QEPV in Caco-2 cell model. QEPV was not detected in Caco-2 cells by flow cytometry(FCM) method or ultra performance liquid chromatography and quadrupole-time-of-flight mass spectrometer(UPLC-Q-TOF-MS) method. Therefore, QEPV could be absorbed as a bioactive peptide via the paracellular pathway in vitro and in vivo. The in vivo experiments demonstrated QEPV could be absorbed by mice via gastrointestinal and abdominal routes. The efficiency of intestinal absorption was lower than that of peritoneal absorption.

Key words: Key words: bioactive peptide, Caco-2 cell model, mouse model, transepithelial transport

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