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Antioxidative Capacity of Pigments from Pu-erh Tea Based on HepG2 Cell Model

CHEN Yalan1, WANG Xueqing1,*, WANG Yiwen1, ZHENG Ziqing1, YE Meixia1, FU Fangfang1, SONG Wenjun1, WANG Suying1, BAI Xiaoli2, LI Changwen2   

  1. 1. Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China; 2. Yunnan Tasly Deepure Biological Tea Group Co. Ltd., Pu Erh 665100, China
  • Online:2017-01-15 Published:2017-01-16

Abstract: In terms of catalase (CAT) activity and malondialdehyde (MDA) and glutathione (GSH) levels, the antioxidative
capacity of pigments from Pu-erh tea was assessed using HepG2 cell models cultured with normal or oleic acid enriched
medium. The results showed that the antioxidative capacity of Pu-erh tea pigments was significant on the oleic acid-induced
HepG2 cell model in a concentration-dependent manner rather than on the normally cultured ones. The extracellular GSH
content and CAT activity in HepG2 cells induced by oleic acid for 24 h with added Pu-erh tea pigments increased from 0.004 4
to 0.010 3 g/L and from 0.136 to 1.174 U/mL, respectively, and MDA content reduced from 15.146 to 7.635 nmol/mg, almost
reaching the normal values. Therefore, the antioxidative mechanism of Pu-erh tea pigments may be due to enhancing reactive
oxygen species (ROS) scavenging enzyme activities, promoting the synthesis of reducing substances and therefore altering
the status of oxidative stress of HepG2 cells.

Key words: HepG2, antioxidant capacity, cell model, Pu-erh tea, tea pigments

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