Abstract: In this study, a method using SiO2/diol based solid-phase extraction (SPE) and multi-dimensional mass spectrometry-based shotgun lipidomics was developed for studying the phospholipids in muscle tissue of Channa argus. Crude lipids were extracted by the Folch method. Phospholipids were obtained by SiO2/diol-SPE, and it’s the extraction efficiency was compared with that of two other commercial SPE cartridges. The conditions for SiO2/diol-SPE were optimized as follows: loading volume, 100 μL; washing solvent pH 2, and 1 mL of 50% acetonitrile as eluent. The eluate was directly injected into the electrospray ionization (ESI) source. The phospholipid molecular species were identified and quantified using the precursor-ion scan and neutral loss scan modes, and the structures of two fatty acid chains in each species were confirmed by multi-dimensional mass spectrometry. The results showed that a total of 67 phospholipid molecular species were detected, including 18 phosphatidylcholines (PC), 21 phosphatidylethanoamines (PE), 12 phosphatidylinositols (PI), and 16 phosphatidylserines (PS). The phospholipids in C. argus contained abundant unsaturated fatty acyl chains. This analytical method proved to be simple, efficient, and reliable.

Key words: shotgun lipidomics, multi-dimensional mass spectrometry (MD-MS), SiO2/diol, solid-phase extraction (SPE), Channa argus