FOOD SCIENCE ›› 2017, Vol. 38 ›› Issue (2): 164-169.doi: 10.7506/spkx1002-6630-201702027

• Component Analysis • Previous Articles     Next Articles

Separation of L-Epicatechin in Liubao Tea by Molecularly Imprinted Solid Phase Extraction

LIU Boyang, LI Lijun, CHENG Hao, HUANG Wenyi, FENG Jun, KONG Hongxing   

  1. 1. Guangxi Key Laboratory of Green Processing of Sugar Resources, College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou 545006, China; 2. College of Medical, Guangxi University of Science and Technology, Liuzhou 545005, China
  • Online:2017-01-25 Published:2017-01-16

Abstract: Molecularly imprinted polymers (MIPs) were synthesized by in situ polymerization using L-epicatechin as the template molecule, acrylamide (AM) as the functional monomer, azobisisobutyronitrile (AIBN) as the initiator, and ethylene glycol dimethacrylate (EGDMA) as the cross-linking agent. Firstly, the specific adsorption capacity of L-epicatechin MIPs was evaluated as a function of the proportions of template molecule, functional monomer, and cross-linking agent. The results showed that when the molar ratio of L-epicatechin to acrylamide to ethylene glycol dimethacrylate was 1:6:40, MIPs exhibited the best adsorption capacity, with template molecule recovery (KMIPs) of 84.62% and specific recognition factor (Q) of 4.55. Scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FT-IR) were used to characterize the MIPs. Finally, we used capillary electrophoresis to detect the effluent and the eluate under optimum conditions. As a result, a molecularly imprinted solid phase extraction-capillary electrophoresis (MISPE-CE) method for the determination of L-epicatechin was developed. The experimental results showed that molecularly imprinted polymers were successfully synthesized with good morphology and specific adsorption characteristics. This method is feasible for the analysis of L-epicatechin in Liubao tea.

Key words: molecular imprinting, L-epicatechin, solid phase extraction, capillary electrophoresis

CLC Number: