Abstract: In this work, the cultured mycelia of Hericium erinaceus were hydrolyzed by 5 mL of a 1% mixture of pectinase and cellulase at a mass ratio of 1:1 in a 50 ℃ water bath for 30 min. In order to increase polysaccharide yield, microwave was used to intensify the hydrolysis of Hericium erinaceus mycelia, and operating parameters such as microwave power, treatment time and material-to-liquid ratio were optimized using one-factor-at-a-time combined with orthogonal array design. The optimal microwave treatment conditions were found to be 500 W, 3 min, and 1:50 (g/mL). Under these conditions, the extraction rate of polysaccharides was 8.01%.

Key words: Hericium erinaceus mycelium, polysaccharides, microwave-assisted enzymatic hydrolysis