FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (4): 209-213.doi: 10.7506/spkx1002-6630-201204044

• Analysis & Detection • Previous Articles     Next Articles

Simultaneous Determination of Carbamate Insecticides and Metabolite Residues in Animal Muscle by High Performance Liquid Chromatography-Tandem Mass Spectrometry

DENG Long,GUO Xin-dong,HE Qiang,YANG Qun-hua,SHEN Yu-dong,WANG Hong   

  1. 1. Key Laboratory of Food Quality and Safety of Guangdong Province, Research Institute of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China;2. Guangzhou Quality Supervision and Testing Institute, Guangzhou 510110, China;3. Guangdong Institute for the Control of Agrochemicals, Guangzhou 510500, China
  • Online:2012-02-25 Published:2012-02-14

Abstract: A high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HPLC-ESI/MS/MS) method for rapid determination of 16 residual analytes including carbamate insecticides and their metabolites in animal muscles was established. Samples were extracted with acetonitrile, and the extract was then condensed, cleaned up, analyzed by reversed phase HPLC-ESI/MS/MS, and quantified by internal standard method. The calibration curves for the analytes exhibited a good linear relationship in the range of 1.0-100 μg/L with correlation coefficient higher than 0.9959. The limits of quantification (LOQ) ranged from 0.5 to 2.5 μg/kg. The recovery rates of the analytes from animal muscle sample across the spike levels of 5.0, 10.0μg/kg and 20 μg/kg were in the range of 71.4%-105.5% with relative standard deviation (RSD) varying from 3.2% to 13.7%. The method was accurate, sensitive, simple and suitable for the simultaneous analysis of  carbamate insecticides and their metabolite residues in animal muscle.

Key words: carbamate, high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HPLC-ESI/MS/MS), animal muscle

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