FOOD SCIENCE ›› 2017, Vol. 38 ›› Issue (8): 43-48.doi: 10.7506/spkx1002-6630-201708008

• Bioengineering • Previous Articles     Next Articles

Investigation of Fermentation Conditions for Fibrinolysin Production from Sea Cucumber Ovum by Bacillus natto and Bioactive Activity of the Fermentation Product

WANG Ting, WEN Zijian, JI Xiaotong, WANG Ling, NIAN Yiying, XUE Peng, SUN Meiling, ZHENG Yingxue,YAO Xishan, LI Dongmei, ZHANG Gongliang, HOU Hongman, SUN Liming,   

  1. 1. School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China; 2. National Engineering Research Center of Seafood, Dalian 116034, China
  • Online:2017-04-25 Published:2017-04-24

Abstract: This study aimed to develop a new functional product by fermenting sea cucumber ovum, a by-product of sea cucumber processing, with Bacillus natto. Fibrinolysin (FIB) activity was measured as an index to optimize the fermentation condition. Results showed that the optimum carbon source was glucose at a concentration of 2%. The optimized results of other fermentation parameters were as follows: initial pH, 7; inoculum amount, 5%; fermentation temperature, 37 ℃; medium loading volume, 20%; solid-to-liquid ratio, 1:30 (g/mL); and rotational speed, 180 r/min. The fermentation supernatant, collected after centrifugation at 7 000 r/min for 20 min, demonstrated FIB activity as high as 6 723 FU/mL and ACE inhibition activity with a high inhibition percentage of 90% at a final protein concentration of 20 mg/mL. The time required for partial activation of thromboplastin and the thrombin time were significantly extended by the supernatant when the protein level was 5–20 mg/mL, and it also decreased FIB content while having no influence on prothrombin time. Furthermore, some proteases in the supernatant were observed by zymography. Therefore, the fermentation product of sea cucumber ovum showed relatively strong thrombolytic, antihypertensive and anticoagulation activities, demonstrating a potential auxiliary therapeutic agent for cardiovascular disease. Hence, it deserved further development.

Key words: sea cucumber ovum, Bacillus natto, submerged fermentation, bioactivity

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