FOOD SCIENCE ›› 2017, Vol. 38 ›› Issue (8): 171-178.doi: 10.7506/spkx1002-6630-201708027

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Qualitative Analysis of Polysaccharides from Different Tissues of Turbot (Scophthalmus maximus) by HPLC-MS/MS

GUO Li,, LIU Bin,, WEN Chengrong,, SONG Shuang,, ZHU Beiwei,   

  1. 1. School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China; 2. College of Food and Pharmaceutical Engineering, Suihua University, Suihua 152061, China; 3. National Engineering Research Center of Seafood, Dalian 116034, China; 4. National and Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian 116034, China
  • Online:2017-04-25 Published:2017-04-24

Abstract: Polysaccharides from bone, muscle, and skin of turbot (Scophthalmus maximus) were extracted by sequential hydrolysis with trypsin and papain followed by alkali extraction or the reverse sequence for functional food application. The disaccharide fragments from turbot polysaccharides were analyzed using high performance liquid chromatographytandem mass spectrometry (HPLC-MS/MS) after trifluoroacetic acid (TFA) hydrolysis and 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization. The crude polysaccharides from fish bone produced by enzymatic pretreatment and alkali extraction contained the highest amounts of neutral polysaccharides (6.79 mg/g dried weight) and sulfated polysaccharides (3.81 mg/g dried weight). The uronic acid polysaccharides (UACPs) from fish bone were identified mainly as chondroitin sulfate (CS) by comparing their retention time and mass spectral data with those of standards, the UACPs from fish mainly included hyaluronic acid and chondroitin sulfate, and the UACPs from fish muscle mainly consisted of hyaluronic acid, heparin, chondroitin sulfate, a polysaccharide comprised of GlcA(1→2)-Man repeated units (AGSP), and an unknown polysaccharide UACP1 with repeats of uronic acid linked to hexose.

Key words: turbot, uronic acid-containing polysaccharide, HPLC-MS/MS

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