FOOD SCIENCE ›› 2017, Vol. 38 ›› Issue (8): 239-243.doi: 10.7506/spkx1002-6630-201708037

• Safety Detection • Previous Articles     Next Articles

ELISA Determination of Enrofloxacin

YANG Yi, MENG Kunjie, ZHAO Hongqiong, Mikeremu·SHAYIBUZHATI, ZHANG Xiaoying,   

  1. 1. College of Veterinary Medicine, Xinjiang Agricultural University, ürümqi 830052, China; 2. College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China
  • Online:2017-04-25 Published:2017-04-24

Abstract: An indirect competitive enzyme-linked immunosorbent immunoassay (ic-ELISA) based on chicken IgY was developed and validated for the detection of enrofloxacin (ENR) residues in animal-derived food samples. The ENR immunogen and coating antigen were prepared by the active ester method and confirmed by UV spectroscopy for further detection and immunization into laying hens. IgY antibodies were extracted with PEG-6000 precipitation. The titer of anti- ENR IgY attained its peak (1:32 000) after the fifth booster immunization. Checkerboard titration showed that 1:64 000 dilution of anti-ENR IgY could give OD around 1.0 at 38 ng/mL ENR-OVA coating concentration. The IC50 of the ELISA for ENR was 18.207 ng/mL and the regression curve equation was y = 0.891 1?0.016 5x (R2 = 0.990). The study demonstrated that anti-ENR IgY antibody was feasible for the detection of ENR in animal-derived food samples.

Key words: enrofloxacin, IgY, antibiotic residues, indirect competitive enzyme-linked immunosorbent immunoassay (ic-ELISA)

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