Study on Antioxidation and anti melanoma of ginseng hairy root

Abstract

Abstract: Panax ginseng hairy root was used as the experimental material, and the antioxidant capacity was determined by three methods: Cu2+ chelating ability, polyphenol oxidase activity and total antioxidant capacity，Cell apoptosis and cell cycle were determined by flow cytometry.The results showed that: with the increase of concentration, the Cu2+ chelating rate of ginseng root was gradually increased, and there was no significant difference between VC at 100 mg/mL;At 40、60、80、100 mg/ml VC polyphenol oxidase activity and FG showed no significant difference;The total antioxidant capacity of ginseng hairy roots was concentration dependent and reached 0.8774 mmolFeSO4/g at 100 mg/mL.Compared with the control group, the apoptosis rate of FG group was significantly , S、G2/M phase increased, and the proportion of G0/G1 phase decreased.Conclusion: Panax ginseng hairy root has antioxidant, anti melanoma effect.

Key words: Ginseng hairy root, antioxidant, Melanoma, Apoptosis, cell cycle

CLC Number:

R285.5

References

[1] Oliveira S, Coelho P, Prudêncio C, et al.Melanoma and obesity: Should antioxidant vitamins be addressed?[J]. Life Sciences, 2016, 165:83-90. [2]Burgeiro A, Bento A C, Gajate C, et al.Rapid human melanoma cell death induced by sanguinarine through oxidative stress[J].European Journal of Pharmacology, 2013, 705(1–3):109-118 [3] Velzen L S V, Wijdeveld M, Black C N, et al.Oxidative stress and brain morphology in individuals with depression, anxiety and healthy controls[J]. Progress in neuro-psychopharmacology& biological psychiatry, 2017, 76(2), 140-144. [4] Zhao W, Feng H, Sun W, et al.Tert-butyl hydroperoxide (t-BHP) induced apoptosis and necroptosis in endothelial cells: Roles of NOX4 and mitochondrion:[J]. Redox Biology, 2017, 11:524-534. [5]Dikalov S.Cross talk between mitochondria and NADPH oxidases[J].Free Radical Biology & Medicine, 2011, 51(7):1289-1301 [6]Liu G S, Wu J C, Tsai H E, et al.Proopiomelanocortin gene delivery induces apoptosis in melanoma through NADPH oxidase 4-mediated ROS generation[J].Free Radical Biology & Medicine, 2014, 70(5):14-22 [7] Baldea I, Olteanu D E, Bolfa P, et al.Efficiency of photodynamic therapy on WM35 melanoma with synthetic porphyrins: Role of chemical structure, intracellular targeting and antioxidant defense[J]. Journal of Photochemistry & Photobiology B Biology, 2015, 151:142-152. [8] 阿萨.白藜芦醇通过调节抗氧化酶活性而呈现抗肿瘤效应[D]. 中南大学, 2013:4-5. [9]Tong L, Chuang C C, Wu S, et al.Reactive oxygen species in redox cancer therapy[J].Cancer Letters, 2015, 367(1):18-25 [10]罗敏, 舒磊, 李映莹, 等.人参总皂苷在实验性肺纤维化小鼠中的抗氧化损伤作用[J].中国药理学与毒理学杂志, 2016, 30(5):511-516 [11]王璐，郜玉钢，王佳宁，等.人参属药用植物鉴定技术研究进展[J].时珍国医国药杂志, 2017, 28(3):694-696 [12] 董妍.糖水煎煮对人参质效影响的研究[D].吉林农业大学, 2016. [13]杨艳文, 孟凡双, 郜玉钢, 等.高效液相色谱法同时测定人参制剂中种人参皂苷方法的建立[J].食品科学, 2016, 37(22):131-135 [14]WU W, LIN QL.Effects of oxidative modification on thermal aggregation and gel properties of soy protein by malondialdehyde[J].Journal of Food Science and Technology, 2014, 51(2):485-493 [15]刘红开, 李放, 张亚宏, 等.不同品种蚕豆种皮中膳食纤维的提取工艺优化及其理化特性[J].食品科学, 2016, 37(16):22-28 [16] 王涵, 赵雨, 刘海龙, 等.不同生长时期人参叶中多酚氧化酶等几种氧化还原酶活力变化研究. 辽宁中医杂志, 2013(8):1676-1678.[] 邢楠楠. 人参10种氧化还原酶的活力研究[D]. 长春中医药大学, 2011. [17] 周瑞.4种滋阴类中药对人参质效的影响及其产品的研制[D].吉林农业大学, 2016. [18] Chung I M, Siddiqui N A, Kim S H, et al.New constituents triterpene ester and sugar derivatives from Panax ginseng Meyer and their evaluation of antioxidant activities[J].Saudi Pharmaceutical Journal, (2017). [19]Jiao L, Li B, Wang M, et al.Antioxidant activities of the oligosaccharides from the roots,flowers and leaves of Panax ginseng CA. Meyer.[J].Carbohydrate Polymers, 2014, 106(12):293- [20]Alencar D B D, Reboucas R H, Santos D R D, et al.Bioactive extracts of red seaweeds Pterocladiella capillacea and Osmundaria obtusiloba(Floridophyceae:Rhodophyta) with antioxidant and bacterial agglutination potential[J].Asian Pacific Journal of Tropical Medicine, 2016, 9(4):372-379 [21] Radice M, Manfredini S, Ziosi P, et al.Herbal extracts, lichens and biomolecules as natural photo-protection alternatives to synthetic UV filters. A systematic review.[J]. Fitoterapia, 2016, 114:144-162. [22]要建超.人参皂苷对小鼠抗疲劳的作用研究[J].中医临床研究, 2016, (29):9-10. [23] Reyes A W B, Hop H T, Arayan L T, et al.The host immune enhancing agent Korean red ginseng oil successfully attenuates Brucella abortus infection in a murine model[J]. Journal of Ethnopharmacology, 2017，198:5-14. [24] Baek K S, Yi Y S, Son Y J, et al.Comparison of the anti-cancer activities of Korean Red Ginseng-derived fractions[J]. 2017. [25]Oh M J, Kim H J, Park E Y, et al.The effect of Korean Red Ginseng extract on rosiglitazone-induced improvement of glucose regulation in diet-induced obese mice:[J].Journal of Ginseng Research, 2017, 41(1):52-59 [26]Jin W L, Mo E J, Ji E C, et al.Effect of Korean Red Ginseng extraction conditions on antioxidant activity,extraction yield,and ginsenoside Rg1 and phenolic content: optimization using response surface methodology[J].Journal of Ginseng Research, 2016, 40(3):229-236 [27]王雪, 黄文华, 张晶, 等.抗黑色素瘤天然药物的研究进展[J].现代药物与临床, 2013, 28(1):83-89 [28]杨志杰，李秀丽.中医药诱导细胞凋亡治疗恶性肿瘤的研究进展[J].中医学报, 2013, 28(176):18- [29] 杨历辉, 李东霞, 苏依拉, 等.小檗碱对人皮肤黑素瘤A375细胞周期相关miRNA的影响[J]. 中国皮肤性病学杂志, 2016(11):1123-1126. [30]Karamitopoulou E，Ｒentsch CA，Markwalder Ｒ，et al.Prognostic sig-nificance of apoptotic cell death in bladder cancer: a tissuemicroarray study on 179 urothelial carcinomas from cystectomy specimens[J].Pathology, 2010, 42(1):37-42 [31]Woo S S, Song J S, Lee J Y, et al.Selection of high ginsenoside producing ginseng hairy root lines using targeted metabolic analysis[J].Phytochemistry, 2004, 65(20):2751-2761 [32] Li J, Liu S, Wang J, et al.Fungal elicitors enhance ginsenosides biosynthesis, expression of functional genes as well as signal molecules accumulation in adventitious roots of Panax ginseng C. A. Mey[J].Journal of Biotechnology, 2016, 239:106-114.