Abstract: A fluorescent magnetic surface molecular imprinting method was used to detect narigin by fluorescence polarization technology. By using TiO2-coated magnetic particles as substrate and methlacrylic acid and acrylamide as monomers, a surface molecular imprinting polymer with both fluorescence and magnetic characteristics was prepared and loaded with fluorescein isothiocyanate. The binding ability of the prepared polymer was tested by fluorescence polarization and ultraviolet (UV) spectrophotometry. Compared two other methods, the fluorescence polarization method was more sensitive, and its limit of detection (LOD) was 0.1 mg/L. The recovery of the fluorescence polarization method was higher than 81.3%. It was shown that the fluorescent magnetic surface molecular imprinting technique could be a new method to quickly and efficiently detect naringin in food.

Key words: naringin, surface molecularly imprinted polymer, magnetic nanoshpere, fluorescence polarization, ultraviolet spectrophotometer