FOOD SCIENCE ›› 2017, Vol. 38 ›› Issue (19): 177-181.doi: 10.7506/spkx1002-6630-201719028

• Nutrition & Hygiene • Previous Articles     Next Articles

Effect of Oligomeric Grape Seed Proanthocyanidins Combined with Cisplatin on the Apoptosis of Human Lung Adenocarcinoma Cell Line A549

WANG Hai, LIAN Yanna, ZHOU You, HUANG Shanwan, GAO Liping*   

  1. Beijing Key Laboratory of Bioactive Substances and Functional Foods, College of Arts and Sciences, Beijing Union University, Beijing 100191, China
  • Online:2017-10-15 Published:2017-09-29

Abstract: Objective: To study the effect of oligomericgrape seed proanthocyanidin (O-GSP) combined with cisplatin (CDDP) on antioxidant defense systems, cell apoptosis and apoptosis-related protein expression in human lung adenocarcinoma cell line A549. Methods: The cells were divided into control, CDDP (5 mg/L), O-GSP (4 mg/L), and O-GSP (4 mg/L) + CDDP (5 mg/L) groups. Malondialdehyde (MDA) content was measured by thiobarbituric acid method. Glutathione (GSH) content was determined by nitrobenzoic acid method. Superoxide dismutase (SOD) activity was examined by xanthine oxidase method. Flow cytometry was used to detect the intracellular ROS content and apoptosis rate. The expression of apoptosis-related proteins such as Bcl-2, Bax and caspase-3 were detected by Western blot. Results: CDDP resulted in a significant increase of MDA and ROS in A549 cells (P < 0.05) and a significant reduction of GSH content and SOD activity (P < 0.05), but O-GSP had no significant effect on the levels of intracellular MDA, GSH and SOD (P > 0.05). Compared with the CDDP group, the levels of MDA, GSH and SOD in the O-GSP + CDDP group were not significantly changed (P > 0.05), while the content of ROS was significantly reduced (P < 0.05). CDDP, O-GSP and their combination could significantly promote cell apoptosis (P < 0.05). The combination of CDDP and O-GSP could increase Bax level and the ratio of Bax to Bcl-2, decrease Bcl-2 expression (P < 0.05), and activate caspase-3. Conclusion: Oligomeric grape seed proanthocyanidins combined with cisplatin can promote the apoptosis of A549 cells, and the apoptosis mechanism was not significantly related to the antioxidant activity of oligomeric grape seed proanthocyanidins but related to the expression of apoptosis-related genes.

Key words: oligomeric grape seed proanthocyanidin (O-GSP), cisplatin, lung adenocarcinoma, cell apoptosis

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