Analysis of Polyphenolic Compounds in Osmanthus fragrans by Extractive Electrospray Ionization Mass Spectrometry

Abstract

Abstract: To establishes a method for the determination of polyphenols in Osmanthus fragrans by extractive electrospray ionization-mass spectrometry (EESI-MS), Osmanthus fragrans var. semperflorens was used as experimental material, the main polyphenolic compounds in the methanol extracts were determined by high performance liquid chromatography (HPLC) and EESI-MS. The results showed that the total phenols and total flavonoids in the methanol extracts of O. fragrans var. semperflorens were 133.38±0.12 mg/g. md and 72.49±0.09 mg/g. md, respectively. Fifteen kinds of polyphenolic compounds were detected in the methanol extracts of the O. fragrans var. semperflorens by HPLC. The contents of myricetin, morin, rutin, naringenin and (+)-catechin were abundant. EESI-MS detection in positive ion mode directly from the O. fragrans var. semperflorens methanol extract detected p-protocatechuic acid, coumaric acid, caffeic acid, epicatechin; negative ion mode to detected cinnamic acid, morin and myricetin. The EESI-MS method in this study has the advantages of less sample consumption, no complex separation, pretreatment and rapid analysis. It can be used for the analysis of polyphenols in food.

Key words: Extractive Electrospray ionization-Mass spectrometry, Total phenols, Total flavonoids, Polyphenol, Osmanthus fragrans

CLC Number:

References

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