Application of Immobilized Tannase in Catalytic Tannin Hydrolysis of Lonicera Edulis

Abstract

Abstract: The optimum conditions for the immobilization of tannins by tannin microspheres were studied by using the tannin derived from Aspergillus niger as raw material,and study on the immobilized enzyme in Lonicera edulis fruit juice colored hydrolysis ability.The result shows,the optimum process conditions for immobilized enzyme were enzyme concentration 0.03mg/mL, glutaraldehyde mass fraction 0.10%, pH 5.4, and immobilization time 40min. Under this condition, the recovery rate of enzyme activity was 71.78%, and the specific activity was 724.72U/mg.The SEM and IR spectra showed that the tannin was immobilized on tannin microspheres.ΔE * ab was 3.49 when the amount of immobilized enzyme was 4 mg;Coloring ability significantly weakened.Microscopic observation showed that the juice treated with the immobilized enzyme could significantly reduce the coloring ability of the pig mucosa.

Key words: tannase, immobilized, Lonicera edulis, colour

CLC Number:

TS27

Jing-Nan Xie. Application of Immobilized Tannase in Catalytic Tannin Hydrolysis of Lonicera Edulis[J]. FOOD SCIENCE, 2018, 39(16): 0-0.

References

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