FOOD SCIENCE ›› 2018, Vol. 39 ›› Issue (16): 0-0.

• Bioengineering •     Next Articles

Comparative Analysis on the Difference in Carbon Sources Use of the Dominant Spoilage Bacteria in Fresh Pseudosciaena crocea

  

  • Received:2017-07-20 Revised:2018-06-07 Online:2018-08-25 Published:2018-08-17
  • Contact: QUAN YOU GUO E-mail:dhsguoqy@163.com

Abstract: The aim is to explore the differences of the carbon source utilization and kinetics parameters of Dominant Spoilage Bacteria under different temperatures of fresh Pseudosciaena crocea.Under the condition of 5, 25 ° C degrees, determined the shelf life by sensory, physical and chemical indicators, first used the MIDI ststem to identified the spoilage bacteria, and than determined the dominant spoilage bacteria by the microbial succession, and used 16 sRNA identification to identified the dominant Spoilage bacteria. Biolog system was applied to the determination of the difference of the carbon source utilization, while Gompertz equation was used to fit the growth curve and solved the kinetic parameters. Results showed: the shelf life of fresh Pseudosciaena crocea at 5°C were 9.0±1.0,while under 25°C the shelf life was 1.0±0.11 days, and the dominant Spoilage Bacteria were Shewanella putrefaciens and Vibrio fluvirus. The average carbon use efficiency at 5°C was less than which at 25°C , and the average well color development (AWCD) of Shewanella putrefaciens at 5°C was higher than Vibrio fluvirus, while at 25°C which was lower than Vibrio fluvirus. At 5°C Shewanella putrefaciens mainly use monosaccharides, polysaccharides, carboxylic acids, esters while Vibrio fluvirus mainly used the monosaccharides, polysaccharides, amino acids and carboxylic acids and under 25°C Shewanella putrefaciens and Vibrio fluvirus mainly used the monosaccharides, polysaccharides, amino acids and carboxylic acids; Conclusion: The temperature effected the utilization of carbon source of spoilage bacteria and the carbon source utilization of different spoilage bacteria are different. By adjusting the temperature, change the substrate carbon source and other environmental factors can achieve the purpose of antibacterial, thereby extending the shelf life of fresh Pseudosciaena crocea

Key words: fresh Pseudosciaena crocea, dominant spoilage bacteria, carbon source utilization

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