Identification of Multi-Toed Beijing-You Chicken by PCR

doi:10.7506/spkx1002-6630-201724037

Abstract

Abstract: Beijing-you chicken is one of the precious local breeds in China. In the natural population, there are some multi-toed individuals because of the mutation of C-G base pair to an A-U pair at the 246 bp site in the LMBR1 gene. In this study, the genotype of multi-toed Beijing-you chicken was found to be different from that of Huadu Arbor Acres (AA) broiler, and the mutated base pair (A-U or C-G) was determined by PCR sequencing and restriction fragment polymorphism (RFLP) so that the breeds could be accurately discriminated from each other.

Key words: Beijing-you chicken, Huadu Arbor Acres broiler, polydactly, PCR sequencing, restriction fragment polymorphism

CLC Number:

TS253.1

CHEN Jingru, XIA Haijian, ZHANG Zebin, QU Lujiang, LIU Yi, LI Xingmin. Identification of Multi-Toed Beijing-You Chicken by PCR[J]. FOOD SCIENCE, 2017, 38(24): 229-234.

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Identification of Multi-Toed Beijing-You Chicken by PCR

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