Abstract: Objective: To investigate the effect of green tea polyphenols (GTP) on the serum level of uric acid in fructoseinduced hyperuricemic rats, and explore the potential mechanism. Methods: Rats were fed with 10 g/100 mL fructose solution for 8 weeks to induce hyperuricemia. Meanwhile, allopurinol (API) at 4 mg/(kg·d) and GTP at 75, 150 or 300 mg/ (kg·d) were intragastrically administered separately to the rats once daily during the fifth to eighth week. Uric acid level in serum was examined by the phosphotungstic acid method. Meantime, the activity and expression of xanthine oxidase (XOD) in liver were tested. In addition, the expression of urate transporter (UAT), urate-anion transporter (URAT) 1 and glucose transporter (GLUT) 9 in kidney were analyzed. Results: 150、300 mg/(kg·d) GTP significantly decreased the serum level of uric acid in fructose-induced hyperuricemic rats (P < 0.05, P < 0.01). At the same time, 150、300 mg/(kg·d) GTP markedly reduced the activity and expression of XOD in the live of hyperuricemic rats (P < 0.05, P < 0.01). Finally, GTP markedly enhanced UAT expression and reduced the expression of URAT1 and GLUT9 in the kidney of hyperuricemic rats (P < 0.05, P < 0.01). Conclusion: 150、300 mg/(kg·d) GTP can decrease the serum level of uric acid in fructose-induced hyperuricemic rats through both reducing uric acid production and promoting uric acid excretion.

Key words: green tea polyphenols, fructose, hyperuricemia, xanthine oxidase, urate transporters