Abstract: Herein, we evaluated the effect of active peptide-N as a new anti-aging agent on aging model mice. For this purpose, an aging model was established by subcutaneously injecting mice with D-galactose (200 mg/(kg·d)) once daily for nine weeks. Thereafter, the mice was separately orally administered with multiple emulsion containing active peptide-N at doses of 2.5, 25.0 and 125.0 μg/ (kg·d). The open field and the Morris water maze test were used to evaluate the locomotor activity and learning and memory ability of mice. Furthermore, glutathione peroxidase (GSH-Px) activity and malondialdehyde (MDA) content in the serum, and acetyl cholinesterase (AChE) activity in the brain tissue of mice were measured. The results showed that active peptide-N multiple emulsion could significantly improve the locomotor activity and learning and memory ability of D-galactose-induced aging mice. The activity of serum GSH-Px was improved by 28.38% (P < 0.05), the concentration of serum MDA was decreased by 26.19% (P < 0.05), and the brain AChE activity was increased by 23.26% in the treated groups (P < 0.05) compared with the aging model group. These results demonstrate that active peptide-N multiple emulsion has an obvious anti-aging effect in D-galactose-induced aging mice.

Key words: active peptide-N multiple emulsion, D-galactose, anti-aging, learning and memory