Effect of Sun-Drying on Potential Allergenicity of Peanut Allergens

doi:10.7506/spkx1002-6630-201803008

FOOD SCIENCE ›› 2018, Vol. 39 ›› Issue (3): 49-54.doi: 10.7506/spkx1002-6630-201803008

• Basic Research • Previous Articles Next Articles

Effect of Sun-Drying on Potential Allergenicity of Peanut Allergens

CHANG Xuejiao1,2, LI Kun1,3, ZHANG Ying1,2, CHEN Hongbing1,4, WU Zhihua1,4,*

1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; 2. School of Food Science and Technology, Nanchang University, Nanchang 330031, China; 3. School of Resources Environment and Chemical Engineering, Nanchang University, Nanchang 330031, China; 4. Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China

Online:2018-02-15 Published:2018-01-30

Abstract

Abstract: Peanut is one of the eight major food allergens, and its allergic reaction is usually life-long. The drying is an essential step of peanut processing. In the present study, peanut allergens were extracted and studied after drying in the sun with or without shells. The amounts and compositions of peanut allergens were measured by the Kjeldahl method, bicinchoninic acid method and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Besides, the structural change of peanut proteins was characterized by circular dichroism, ultraviolet scanning spectroscopy, and the potential allergenicity of allergens was quantified by serum immunoglobulin E (IgE) binding capacity. The results showed that after sun-drying, IgE binding capacity of peanut proteins increased significantly. The secondary structure of peanut proteins dried with shells was more orderly than that of peanut proteins dried without shells, with the tertiary structure being more compact. The peanut proteins dried with shells had higher potential allergenicity, which may be due to their loose structure.

Key words: sun-drying treatment, peanut protein, potential allergenicity, allergen

CLC Number:

TS255.1

CHANG Xuejiao, LI Kun, ZHANG Ying, CHEN Hongbing, WU Zhihua. Effect of Sun-Drying on Potential Allergenicity of Peanut Allergens[J]. FOOD SCIENCE, 2018, 39(3): 49-54.

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Effect of Sun-Drying on Potential Allergenicity of Peanut Allergens

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