FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (11): 265-269.doi: 10.7506/spkx1002-6630-201111054

• Nutrition & Hygiene • Previous Articles     Next Articles

Effect of Corn Peptides on Alcohol Dehydrogenase Activity in Live of Mice after Drinking and Its Anti-alcohol Mechanism

GUO Hui1,2,HE Hui1,*,HAN Ying1,HUANG Wen-hao1,ZHANG Xiao-bo1   

  1. 1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 2. Angel Yeast Co. Ltd., Yichang 443003, China
  • Online:2011-06-15 Published:2011-05-13

Abstract: In order to explore the anti-alcohol mechanism of corn peptides (CP) on heavy-drinking mice, blood alcohol concentration (BAC) was determined by gas chromatography (GC), ADH activity in liver was detected by NAD+ method and the amino acid composition of CP was analyzed. The results showed that CP could activate ADH in liver in a dose-dependent way. CP at the dose of 600 mg/kg ·bw could significantly increase ADH activity in mice administered with alcohol (P < 0.01), result in an ADH activation rate of up to 30.1%, and remarkably reduce BAC in mice (P< 0.01). The scavenging rate of BAC was positively correlated with the dose of CP. The scavenging rate of BAC and the ADH activity in mice from CP group were remarkably higher than that in mice from alcohol model group after the administration of alcohol for 20-200 min. Compared with the model group, the scavenging rate of BAC in mice from the CP group was significantly different during alcohol administration for 20-120 min (P< 0.05 or P< 0.01). Amino acid analysis showed that CP had high hydrophobicity. The highest hydrophobic fraction was the most potent hydroxyl radical scavenger with scavenging rate of 83.05%. Therefore, CP can continuously activate ADH in liver, which likely to be related to hydrophobic short peptides in corn.

Key words: corn peptides (CP), anti-alcohol, alcohol dehydrogenase (ADH), blood alcohol concentration (BAC), hydrophobic

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