Abstract Pinus?protein?peptides?from?protein?of?Pinus?koraiensis?Sieb.?et?Zucc. were?prepared?by?enzymolysis?of?
pepsin?and?trypsin,?and?further?purified?by?ultrafiltration,?SP?Sephadex?C-25?and?Sephadex?G-25. This study screened the?anti-oxidative?Pinus?protein peptides?fraction (PDⅡ),?mensurated the molecular weight distribution of PD II, and conducted?anti-fatigue?test in?vivo.The?results?showed?that,?the molecular weight distribution of the fraction PDⅡ was 500-1100Da, declaring the fraction had a strong antioxidant effect. Different doses of PDⅡcould?keep?the?blood glucose?level, increase?the?glycogen?storage?capacity?and?decrease?the?content?of?serum?
urea?nitrogen,?malondialdehyde?and?blood?lactate?acid. Compared with the blank group, it reached a very significant level (P<0.01), which?indicated?that?the fraction?PDⅡ?isolated?from?the?Pinus?protein?had?both?anti-
Received: 14 December 2017
Published: 22 January 2019
ZHENG Yuan-Yuan,JING Jing,WANG Zhen-Yu, et al. Isolation, Purification and Antioxidant Activity in Vitro and Anti - fatigue in vivo of protein peptide of Pinus koraiensis Sieb. et Zucc.[J]. , 0, (): 0-0.