FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (17): 225-229.doi: 10.7506/spkx1002-6630-201117046

• Bioengineering • Previous Articles     Next Articles

Prediction of B-cell Linear Epitopes in Egg Allergen Ovotransferrin

TONG Ping1,2，GAO Jin-yan3，CHEN Hong-bing1,2,*，LI Xin1,2，JIAN Shan1,2，ZHANG Yin1,2   

  1. (1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China； 2. Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China； 3. Department of Food Science, School of Life Sciences and Food Engineering, Nanchang University, Nanchang 330047, China)
  • Published:2011-08-30

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Abstract: In order to predict B-cell linear epitopes of egg allergen ovotransferrin (OVT), the antigen index, hydrophilicity, and surface accessibility and flexibility of egg OVT were predicted using Jameson-Wolf, Kyte-Doolittle, Emini and Karplus-Schulz methods, respectively. The secondary structure of OVT was predicted by Chou-Fasman and Deleage-Roux methods based on the amino acid sequence of egg OVT derived from Genbank. Based on the above comprehensive analysis, 7 possible B-cell linear epitope candidates in egg OVT were predicted to locate in the regions of 174－181, 215－222, 231－240, 288－294, 332－344, 415－429 and 547－555. These results are useful for fine mapping of B-cell epitopes in egg OVT.

Key words: ovotransferrin, B-cell linear epitope, egg allergy, prediction

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