FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (19): 170-173.doi: 10.7506/spkx1002-6630-201119037

• Bioengineering • Previous Articles     Next Articles

Effect of Propyl p-Hydroxybenzoate on SOD Gene Expression of in Drosophila melanogaster

JIANG Fang-ping,YING Qiong-qiong,GU Wei*   

  1. (College of Life Science, Shaanxi Normal University, Xi,an 710062, China)
  • Published:2011-10-12

Abstract: In order to explore the effect of propyl p-hydroxybenzoate (PP) on superoxide dismutase (SOD) gene expression in Drosophila melanogaster, PP was used to feed Drosophila melanogaster at the concentrations of 0, 0.20, 0.50, 1.00 g/L and 2.00 g/L for 10 consecutive days, respectively. Total RNA was extracted from Drosophila melanogaster and RT-PCR was used to investigate the impact of PP on mRNA expression level of SOD gene in Drosophila melanogaster. The results suggested that PP could significantly suppress Cu, Zn-SOD and Mn-SOD in comparison with the control group (P<0.05). As the PP concentration increased, the expression level of Cu, Zn-SOD gene in female flies and Mn-SOD gene in males revealed a decrease; however, the expression level of Mn-SOD gene in female flies exhibited an obvious improvement. These results indicated that the toxicological effect of PP may be related to the inhibition of SOD gene expression in Drosophila melanogaster, which shows differences between males and females.

Key words: propyl p-hydroxybenzoate (PP), Grosophila melanogaster, superoxide dismutase (SOD), gene expression

CLC Number: