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• Food Engineering •     Next Articles

Effect of Electron Beam Irradiation on Immunoreactivity and Conformation of Tropomyosin from Shrimp (Solenocera melantho)

Guan Aiyan 2, Jin-Jie ZHANG2, Qiao-Ming LOU   

  • Received:2018-01-25 Revised:2018-11-05 Online:2019-02-15 Published:2019-03-05

Abstract: To illustrate the relationship between the conformation and potential allergenicity of shrimp tropomyosin (TM) after electron beam (EB) irradiation, TM extracted from Solenocera melantho was irradiated with different EB doses (0, 1, 3, 5, 7, 9 kGy). The secondary structure and surface hydrophobicity of TM were analyzed by circular dichroism (CD) and fluorescence spectrum, and the IgG binding capacity of TM was determined using western blot and indirect enzyme linked immunosorbent assay (ELISA), respectively. The results showed that EB irradiation can reduce the content of TM. EB irradiation changed the percentage of secondary structure in TM. With the increase of irradiation dose, the α-helix content decreased, while the β-sheet, random coil contents and the surface hydrophobicity of TM increased, meanwhile the binding capacity of TM to IgG decreased. It is demonstrated that EB irradiation can decrease the TM immunoreactivity by breaking its conformational structure. The results provide a theoretical basis for the application of EB irradiation to decrease the potential allergenicity of Solenocera melantho meat.

Key words: shrimp, tropomyosin (TM), electron beam irradiation, immunoreactivity, protein conformation

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