Screening for Protential Probiotics based on High α-glucosidase Inhibitory Activity

Abstract

Abstract: The present study was designed to screen for potential probiotics based on high alpha-glucosidase inhibitory activity from cell-free excretory supernatants (CFS) and cell-free extracts (CFE) of 77 probiotic strains. Meanwhile, probiotic properties such as tolerance to the low pH, simulated saliva (SS), simulated gastric juice (SGJ), simulated intestinal juice (SIJ), transporting, bile salts tolerance as well as adhesion to HT-29 cell assays were also examined. Most cell-free excretory supernatants (CFS) showed alpha-glucosidase inhibition, ranging from 2.53% to 15.76%, but all cell-free extracts (CFE) did not. The strain ST-2, 1.1881, GS-3 and BLP12 showed strong ability of α-glucosidase inhibition. The strain ST-2 showed 75.97% survival rate after 3 h of incubation at pH 2.0, and the strain ST-2 expressed strong adhesive abilities to HT-29 cells; the strain GS-3 showed a survival rate of 89.49% after the complete gastrointestinal transit. Principal component analysis (PCA) showed that the strain BLP12 displayed the best combination characteristics: its alpha-glucosidase inhibition rate was 15.10%; the survival rate of resistance to pH 2.0 was 71.04%; its survival rate was 77.14% in the presence of bile salts (2.0%); the transporting survival rate of the strain BLP12 through SS/SGJ/SIJ was 88.27%; but the rate of adhesion to HT-29 cell was only 1.93%. In general, BLP12 display strong adaptability to simulated environment. BLP12 strain was identified as Lactobacillus plantarum by 16S rDNA sequencing methods. It could be used as potential anti-diabetic probiotics in the development of functional food.

Key words: α-glucosidase, probiotics, inhibition, probiotic properties, screen for

CLC Number:

TS218

References

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