Optimization of Critical Medium Components for Phycocyanin Production by Recombinant Escherichia coli Using Response Surface Methodology

doi:10.7506/spkx1002-6630-201816024

Abstract

Abstract: The critical medium components for phycocyanin production by recombinant Escherichia coli were selected and optimized using response surface methodology. The medium components with significant influence on phycocyanin yield were identified using a fractional factorial design and their levels were optimized using response surface methodology. Peptone, yeast extract and K2HPO4·3H2O were recognized as the critical medium components, and their optimal levels were determined as 23.8, 13.4 and 9.4 g/L, respectively, which gave a phycocyanin yield of 15.28 mg/L. This study can pave a good foundation for future production and use of phycocyanin.

Key words: recombinant E. coli, phycocyanin, fermentation, response surface methodology, optimization

CLC Number:

Q815

YU Ping, XU Chaochao, ZHU Pengzhi. Optimization of Critical Medium Components for Phycocyanin Production by Recombinant Escherichia coli Using Response Surface Methodology[J]. FOOD SCIENCE, 2018, 39(16): 161-167.

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Optimization of Critical Medium Components for Phycocyanin Production by Recombinant Escherichia coli Using Response Surface Methodology

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